A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30377346.
- Also identified by DOI 10.1038/s41592-018-0173-1 and PMC identifier 6214191.
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Abstract
We report a fully automated procedure for the optimization and interpretation of reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as phenix.map_to_model. We applied our approach to 476 datasets with resolution of 4.5 Å or better, including reconstructions of 47 ribosomes and 32 other protein-RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 Å or better and 47% for those at resolutions worse than 3 Å.
Medical subject headings
- Cryoelectron Microscopy
- Models, Molecular
- RNA
- RNA-Binding Proteins
- Ribosomes