FLIRT: fast local infrared thermogenetics for subcellular control of protein function.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30377360.
- Also identified by DOI 10.1038/s41592-018-0168-y and PMC identifier 6295154.
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Abstract
FLIRT (fast local infrared thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature-sensitive mutant proteins. We demonstrate that FLIRT can control temperature-sensitive proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in Caenorhabditis elegans with cell-specific and even subcellular precision.
Medical subject headings
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Genetic Techniques
- Infrared Rays
- Molecular Imaging
- Mutation
- Temperature