Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30377362.
- Also identified by DOI 10.1038/s41592-018-0178-9.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Anti-CRISPR proteins are powerful tools for CRISPR-Cas9 regulation; the ability to precisely modulate their activity could facilitate spatiotemporally confined genome perturbations and uncover fundamental aspects of CRISPR biology. We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR-Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.
Medical subject headings
- Biosensing Techniques
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Gene Editing
- Optogenetics
- Phototropins
- Protein Engineering