Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30377363.
- Also identified by DOI 10.1038/s41592-018-0171-3 and PMC identifier 6394230.
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Abstract
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.
Medical subject headings
- Glutamic Acid
- Green Fluorescent Proteins
- Microscopy, Fluorescence
- Neurons
- Retina
- Visual Cortex