The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.
basic_science · Level V
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- Record sourced from PubMed, PMID 30377366.
- Also identified by DOI 10.1038/s41592-018-0180-2.
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Abstract
We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
Medical subject headings
- Crystallography, X-Ray
- Hydrolases
- Protein Conformation
- Rhodopseudomonas
- Synchrotrons