Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30397326.
- Also identified by DOI 10.1038/s41592-018-0177-x and PMC identifier 6405223.
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Abstract
Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.
Medical subject headings
- Brain
- Imaging, Three-Dimensional
- Microscopy, Electron
- Microscopy, Fluorescence
- Single-Domain Antibodies