Near-infrared STED nanoscopy with an engineered bacterial phytochrome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30420676.
- Also identified by DOI 10.1038/s41467-018-07246-2 and PMC identifier 6232180.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.
Medical subject headings
- Bacterial Proteins
- Microscopy, Fluorescence
- Nanotechnology
- Phytochrome
- Protein Engineering