Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30446673.
- Also identified by DOI 10.1038/s41467-018-07244-4 and PMC identifier 6240104.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy, Fluorescence
- Microscopy, Interference
- Microtubules
- Mitochondria
- Osteoblasts