Automated sequential chromogenic IHC double staining with two HRP substrates.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30458050.
- Also identified by DOI 10.1371/journal.pone.0207867 and PMC identifier 6245840.
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Abstract
Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.
Medical subject headings
- Horseradish Peroxidase
- Immunohistochemistry
- Staining and Labeling