Splicing factors Sf3A2 and Prp31 have direct roles in mitotic chromosome segregation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30475206.
- Also identified by DOI 10.7554/eLife.40325 and PMC identifier 6287947.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Several studies have shown that RNAi-mediated depletion of splicing factors (SFs) results in mitotic abnormalities. However, it is currently unclear whether these abnormalities reflect defective splicing of specific pre-mRNAs or a direct role of the SFs in mitosis. Here, we show that two highly conserved SFs, Sf3A2 and Prp31, are required for chromosome segregation in both <i>Drosophila</i> and human cells. Injections of anti-Sf3A2 and anti-Prp31 antibodies into <i>Drosophila</i> embryos disrupt mitotic division within 1 min, arguing strongly against a splicing-related mitotic function of these factors. We demonstrate that both SFs bind spindle microtubules (MTs) and the Ndc80 complex, which in Sf3A2- and Prp31-depleted cells is not tightly associated with the kinetochores; in HeLa cells the Ndc80/HEC1-SF interaction is restricted to the M phase. These results indicate that Sf3A2 and Prp31 directly regulate interactions among kinetochores, spindle microtubules and the Ndc80 complex in both <i>Drosophila</i> and human cells.
Medical subject headings
- Drosophila Proteins
- Drosophila melanogaster
- Eye Proteins
- Mitosis
- Nuclear Proteins
- RNA Splicing Factors