Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30476132.
- Also identified by DOI 10.1093/infdis/jiy627 and PMC identifier 6420172.
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Abstract
We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of >6 μg/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models.
Medical subject headings
- Antibodies, Monoclonal, Humanized
- Antigens, Surface
- Bacterial Outer Membrane Proteins
- Bacterial Vaccines
- DNA, Bacterial
- Lipoproteins
- Lyme Disease