Termite mounds mitigate half of termite methane emissions.

Abstract

Termites are responsible for ∼1 to 3% of global methane (CH₄) emissions. However, estimates of global termite CH₄ emissions span two orders of magnitude, suggesting that fundamental knowledge of CH₄ turnover processes in termite colonies is missing. In particular, there is little reliable information on the extent and location of microbial CH₄ oxidation in termite mounds. Here, we use a one-box model to unify three independent field methods-a gas-tracer test, an inhibitor approach, and a stable-isotope technique-and quantify CH₄ production, oxidation, and transport in three North Australian termite species with different feeding habits and mound architectures. We present systematic in situ evidence of widespread CH₄ oxidation in termite mounds, with 20 to 80% of termite-produced CH₄ being mitigated before emission to the atmosphere. Furthermore, closing the CH₄ mass balance in mounds allows us to estimate in situ termite biomass from CH₄ turnover, with mean biomass ranging between 22 and 86 g of termites per kilogram of mound for the three species. Field tests with excavated mounds show that the predominant location of CH₄ oxidation is either in the mound material or the soil beneath and is related to species-specific mound porosities. Regardless of termite species, however, our data and model suggest that the fraction of oxidized CH₄ (<i>f</i>_ox) remains well buffered due to links among consumption, oxidation, and transport processes via mound CH₄ concentration. The mean <i>f</i>_ox of 0.50 ± 0.11 (95% CI) from in situ measurements therefore presents a valid oxidation factor for future global estimates of termite CH₄ emissions.