Efficient scarless genome editing in human pluripotent stem cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30504872.
- Also identified by DOI 10.1038/s41592-018-0212-y.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR-Cas9-mediated homologous recombination with positive-negative selection of edited clones to generate scarless genetic changes in hPSCs.
Medical subject headings
- CRISPR-Cas Systems
- Embryonic Stem Cells
- Gene Editing
- Genome, Human
- Homologous Recombination
- Pluripotent Stem Cells
- RNA, Small Interfering