Regulating Osteogenic Differentiation by Suppression of Exosomal MicroRNAs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30520703.
- Also identified by DOI 10.1089/ten.TEA.2018.0257.
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Abstract
We investigated the role of exosomes in osteogenesis and the use of miRNA inhibitor-transfected exosomes to control osteogenic differentiation. RNA-sequencing (RNA-seq) of exosomal miRNAs revealed that growth condition of milieu of preosteoblast exosomes harbors high levels of let-7, which plays a critical role in osteogenesis regulation. We modified exosomes by transfecting let-7 inhibitor into exosomes under growth condition in MC3T3-E1 cells and revealed that exosomes whose let-7 was inactivated by engineering lost the ability to recover osteogenic differentiation. Genetically modified exosomes may serve as powerful biomaterials for developmental control, including of osteogenesis regulation.
Medical subject headings
- Cell Differentiation
- Exosomes
- MicroRNAs
- Osteogenesis