Effect of radiotherapy on the chemical composition of root dentin.

Abstract

The radiotherapy can directly affect the bond strength of the adhesive materials, interfering in the prognosis of restorative treatments, which may be caused by chemical changes in dentin structure. Twenty inferior homologues premolars were distributed in 2 groups (in vitro study) (n = 10): nonirradiated and irradiated. The specimens were submitted to the analysis of phosphate (ν₁ PO₄^3- ;ν₂ PO₄^3- ;ν₄ PO₄^3- ), carbonate (ν₃ CO₃^2- ), amide I, CH₂ , amide III, and amide I/III ratio by confocal Raman spectroscopy. Data were submitted to statistical analysis (T test, P < .05). In intracanal dentin, the irradiated group had lower ν₄ PO₄^3- values (1.23 ± 0.06) compared to nonirradiated group (1.40 ± 0.18) (P < .05), with no difference for ν₁ PO₄^3- and ν₂ PO₄³ peaks (P > .05). The irradiated (1.56 ± 0.06) had lower carbonate, amide III (1.05 ± 0.19), and amide I/III ratio values (0.19 ± 0.06) compared to nonirradiated group (1.42 ± 0.10, 1.28 ± 0.24, and 0.31 ± 0.10, respectively) (P < .05). For medium dentin irradiated group (1.30 ± 0.12) had lower phosphate values compared to nonirradiated group (1.48 ± 0.22) (P < .05). In cementum, there was no statistical difference between the groups. The radiotherapy was able to cause changes in ν₄ PO₄^3- , carbonate, and amide III peaks of root dentin.