Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30559431.
- Also identified by DOI 10.1038/s41592-018-0259-9 and PMC identifier 6378878.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We describe droplet-assisted RNA targeting by single-cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.
Medical subject headings
- Gene Expression Profiling
- Single-Cell Analysis
- Transcriptome