Multiplexed orthogonal genome editing and transcriptional activation by Cas12a.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30559432.
- Also identified by DOI 10.1038/s41592-018-0262-1.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
CRISPR-Cas9-based combinatorial perturbation approaches for orthogonal knockout and gene activation have been impeded by complex vector designs and co-delivery of multiple constructs. Here, we demonstrate that catalytically active CRISPR-Cas12a fused to a transcriptional-activator domain enables flexible switching between genome editing and transcriptional activation by altering guide length. By leveraging Cas12a-mediated CRISPR-RNA array processing, we illustrate that Cas12a-VPR enables simplified multiplexed knockout and transcriptional activation in vitro and in vivo.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing
- Transcriptional Activation