Local mutational diversity drives intratumoral immune heterogeneity in non-small cell lung cancer.
case_series · Level IV
Where this comes from
- Record sourced from PubMed, PMID 30560866.
- Also identified by DOI 10.1038/s41467-018-07767-w and PMC identifier 6299138.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Combining whole exome sequencing, transcriptome profiling, and T cell repertoire analysis, we investigate the spatial features of surgically-removed biopsies from multiple loci in tumor masses of 15 patients with non-small cell lung cancer (NSCLC). This revealed that the immune microenvironment has high spatial heterogeneity such that intratumoral regional variation is as large as inter-personal variation. While the local total mutational burden (TMB) is associated with local T-cell clonal expansion, local anti-tumor cytotoxicity does not directly correlate with neoantigen abundance. Together, these findings caution against that immunological signatures can be predicted solely from TMB or microenvironmental analysis from a single locus biopsy.
Medical subject headings
- Antigens, Neoplasm
- Carcinoma, Non-Small-Cell Lung
- Lung Neoplasms
- T-Lymphocytes
- Tumor Microenvironment