Reliability of human cortical organoid generation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30573846.
- Also identified by DOI 10.1038/s41592-018-0255-0 and PMC identifier 6677388.
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Abstract
The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.
Medical subject headings
- Organoids
- Tissue Engineering