Online photochemical derivatization enables comprehensive mass spectrometric analysis of unsaturated phospholipid isomers.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30622271.
- Also identified by DOI 10.1038/s41467-018-07963-8 and PMC identifier 6325166.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Mass spectrometry-based lipidomics is the primary tool for the structural analysis of lipids but the effective localization of carbon-carbon double bonds (C=C) in unsaturated lipids to distinguish C=C location isomers remains challenging. Here, we develop a large-scale lipid analysis platform by coupling online C=C derivatization through the Paternò-Büchi reaction with liquid chromatography-tandem mass spectrometry. This provides rich information on lipid C=C location isomers, revealing C=C locations for more than 200 unsaturated glycerophospholipids in bovine liver among which we identify 55 groups of C=C location isomers. By analyzing tissue samples of patients with breast cancer and type 2 diabetes plasma samples, we find that the ratios of C=C isomers are much less affected by interpersonal variations than their individual abundances, suggesting that isomer ratios may be used for the discovery of lipid biomarkers.
Medical subject headings
- Breast Neoplasms
- Diabetes Mellitus, Type 2
- Glycerophospholipids
- Point-of-Care Systems