A genetically encoded near-infrared fluorescent calcium ion indicator.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30664778.
- Also identified by DOI 10.1038/s41592-018-0294-6 and PMC identifier 6393164.
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Abstract
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca<sup>2+</sup>) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca<sup>2+</sup> transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca<sup>2+</sup> imaging in combination with other optogenetic indicators and actuators.
Medical subject headings
- Calcium
- Fluorescent Dyes
- Microscopy, Fluorescence
- Spectroscopy, Near-Infrared