Nephron progenitor commitment is a stochastic process influenced by cell migration.

Lawlor, Kynan T; Zappia, Luke; Lefevre, James; Park, Joo-Seop; Hamilton, Nicholas A; Oshlack, Alicia; Little, Melissa H; Combes, Alexander N · Elife · 2019

basic_science · Level V

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Abstract

Progenitor self-renewal and differentiation is often regulated by spatially restricted cues within a tissue microenvironment. Here, we examine how progenitor cell migration impacts regionally induced commitment within the nephrogenic niche in mice. We identify a subset of cells that express <i>Wnt4</i>, an early marker of nephron commitment, but migrate back into the progenitor population where they accumulate over time. Single cell RNA-seq and computational modelling of returning cells reveals that nephron progenitors can traverse the transcriptional hierarchy between self-renewal and commitment in either direction. This plasticity may enable robust regulation of nephrogenesis as niches remodel and grow during organogenesis.

Medical subject headings