FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30688648.
- Also identified by DOI 10.7554/eLife.43146 and PMC identifier 6349402.
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Abstract
The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells in clathrin-coated vesicles and endosomes. AuNPs were present but no longer paired in multivesicular bodies. FGF21 could thus be tracked along the endocytotic pathway. The methods developed here to visualize signaling coupled to endocytosis can be applied to a wide variety of cargo and may be extended to studies of other intracellular transactions.
Medical subject headings
- Cell Membrane
- Endocytosis
- Endosomes
- Fibroblast Growth Factors