Top-Down Garbage Collector: a tool for selecting high-quality top-down proteomics mass spectra.

Lima, Diogo B; Silva, André R F; Dupré, Mathieu; Santos, Marlon D M; Clasen, Milan A; Kurt, Louise U; Aquino, Priscila F; Barbosa, Valmir C et al. · Bioinformatics · 2019

other · Level V

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Abstract

We present the first tool for unbiased quality control of top-down proteomics datasets. Our tool can select high-quality top-down proteomics spectra, serve as a gateway for building top-down spectral libraries and, ultimately, improve identification rates. We demonstrate that a twofold rate increase for two E. coli top-down proteomics datasets may be achievable. http://patternlabforproteomics.org/tdgc, freely available for academic use. Supplementary data are available at Bioinformatics online.

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