Multispectral tracing in densely labeled mouse brain with nTracer.
basic_science · Level V
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- Record sourced from PubMed, PMID 30715234.
- Also identified by DOI 10.1093/bioinformatics/btz084 and PMC identifier 6748755.
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Abstract
This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial. Supplementary data are available at Bioinformatics online.
Medical subject headings
- Software