Resistance gene cloning from a wild crop relative by sequence capture and association genetics.

Arora, Sanu; Steuernagel, Burkhard; Gaurav, Kumar; Chandramohan, Sutha; Long, Yunming; Matny, Oadi; Johnson, Ryan; Enk, Jacob et al. · Nat Biotechnol · 2019

basic_science · Level V

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Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

Medical subject headings