The genetic requirements of fatty acid import by <i>Mycobacterium tuberculosis</i> within macrophages.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30735132.
- Also identified by DOI 10.7554/eLife.43621 and PMC identifier 6368401.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
<i>Mycobacterium tuberculosis</i> (Mtb) imports and metabolizes fatty acids to maintain infection within human macrophages. Although this is a well-established paradigm, the bacterial factors required for fatty acid import are poorly understood. Previously, we found that LucA and Mce1 are required for fatty acid import in Mtb (Nazarova et al., 2017). Here, we identified additional Mtb mutants that have a reduced ability to import a fluorescent fatty acid substrate during infection within macrophages. This screen identified the novel genes as <i>rv2799</i> and <i>rv0966c</i> as be necessary for fatty acid import and confirmed the central role for Rv3723/LucA and putative components of the Mce1 fatty acid transporter (Rv0200/OmamB, Rv0172/Mce1D, and Rv0655/MceG) in this process.
Medical subject headings
- Bacterial Proteins
- Fatty Acids
- Mycobacterium tuberculosis
- Tuberculosis