How a circularized tmRNA moves through the ribosome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30765567.
- Also identified by DOI 10.1126/science.aav9370 and PMC identifier 6440651.
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Abstract
During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.
Medical subject headings
- Protein Biosynthesis
- RNA, Bacterial
- RNA-Binding Proteins
- Ribosomes