Development of a CRISPR/Cas9-based therapy for Hutchinson-Gilford progeria syndrome.

Santiago-Fernández, Olaya; Osorio, Fernando G; Quesada, Víctor; Rodríguez, Francisco; Basso, Sammy; Maeso, Daniel; Rolas, Loïc; Barkaway, Anna et al. · Nat Med · 2019

basic_science · Level V

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Abstract

CRISPR/Cas9-based therapies hold considerable promise for the treatment of genetic diseases. Among these, Hutchinson-Gilford progeria syndrome, caused by a point mutation in the LMNA gene, stands out as a potential candidate. Here, we explore the efficacy of a CRISPR/Cas9-based approach that reverts several alterations in Hutchinson-Gilford progeria syndrome cells and mice by introducing frameshift mutations in the LMNA gene.

Medical subject headings