Universal light-sheet generation with field synthesis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30804550.
- Also identified by DOI 10.1038/s41592-019-0327-9 and PMC identifier 6561754.
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Abstract
We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.
Medical subject headings
- Light
- Microscopy, Fluorescence