Fluid dynamics during bleb formation in migrating cells in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30807602.
- Also identified by DOI 10.1371/journal.pone.0212699 and PMC identifier 6391022.
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Abstract
Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.
Medical subject headings
- Cell Movement
- Cytokinesis
- Cytoplasm
- Germ Cells
- Models, Biological
- Zebrafish