Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30814529.
- Also identified by DOI 10.1038/s41467-019-08880-0 and PMC identifier 6393466.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.
Medical subject headings
- Ribosomal Proteins
- Ribosomes
- Saccharomyces cerevisiae Proteins