metagenomeFeatures: an R package for working with 16S rRNA reference databases and marker-gene survey feature data.

Olson, Nathan D; Shah, Nidhi; Kancherla, Jayaram; Wagner, Justin; Paulson, Joseph N; Corrada Bravo, Hector · Bioinformatics · 2019

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Abstract

SUMMARY: We developed the metagenomeFeatures R Bioconductor package along with annotation packages for three 16S rRNA databases (Greengenes, RDP and SILVA) to facilitate working with 16S rRNA databases and marker-gene survey feature data. The metagenomeFeatures package defines two classes, MgDb for working with 16S rRNA sequence databases, and mgFeatures for marker-gene survey feature data. The associated annotation packages provide a consistent interface to the different databases facilitating database comparison and exploration. The mgFeatures-class represents a crucial step in the development of a common data structure for working with 16S marker-gene survey data in R. AVAILABILITY AND IMPLEMENTATION: https://bioconductor.org/packages/release/bioc/html/metagenomeFeatures.html. SUPPLEMENTARY INFORMATION: Supplementary material is available at Bioinformatics online.

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