Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30846702.
- Also identified by DOI 10.1038/s41467-019-08990-9 and PMC identifier 6405920.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Activated macrophages have the potential to be ideal targets for imaging inflammation. However, probe selectivity over non-activated macrophages and probe delivery to target tissue have been challenging. Here, we report a small molecule probe specific for activated macrophages, called CDg16, and demonstrate its application to visualizing inflammatory atherosclerotic plaques in vivo. Through a systematic transporter screen using a CRISPR activation library, we identify the orphan transporter Slc18b1/SLC18B1 as the gating target of CDg16.
Medical subject headings
- Cation Transport Proteins
- Inflammation
- Macrophage Activation