A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30858599.
- Also identified by DOI 10.1038/s41592-019-0333-y.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity-an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a multiplexed assay that uses metabolism-sensitive staining to report on the killing of antibiotic-tolerant bacteria. This method can be used with diverse bacterial species and applied to genome-scale investigations to identify therapeutic targets against tolerant pathogens.
Medical subject headings
- Anti-Bacterial Agents
- Bacteria
- Drug Resistance, Bacterial
- Escherichia coli
- Microbial Sensitivity Tests