124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30864449.
- Also identified by DOI 10.1021/acs.nanolett.9b00508 and PMC identifier 6463241.
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Abstract
Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.
Medical subject headings
- DNA
- Microscopy, Fluorescence
- Proteins
- RNA