Optogenetic stimulation of G<sub>s</sub>-signaling in the heart with high spatio-temporal precision.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30894542.
- Also identified by DOI 10.1038/s41467-019-09322-7 and PMC identifier 6426906.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The standard technique for investigating adrenergic effects on heart function is perfusion with pharmaceutical agonists, which does not provide high temporal or spatial precision. Herein we demonstrate that the light sensitive G<sub>s</sub>-protein coupled receptor JellyOp enables optogenetic stimulation of G<sub>s</sub>-signaling in cardiomyocytes and the whole heart. Illumination of transgenic embryonic stem cell-derived cardiomyocytes or of the right atrium of mice expressing JellyOp elevates cAMP levels and instantaneously accelerates spontaneous beating rates similar to pharmacological β-adrenergic stimulation. Light application to the dorsal left atrium instead leads to supraventricular extrabeats, indicating adverse effects of localized G<sub>s</sub>-signaling. In isolated ventricular cardiomyocytes from JellyOp mice, we find increased Ca<sup>2+</sup> currents, fractional cell shortening and relaxation rates after illumination enabling the analysis of differential G<sub>s</sub>-signaling with high temporal precision. Thus, JellyOp expression allows localized and time-restricted G<sub>s</sub> stimulation and will provide mechanistic insights into different effects of site-specific, long-lasting and pulsatile G<sub>s</sub> activation.
Medical subject headings
- GTP-Binding Protein alpha Subunits, Gs
- Heart Atria
- Heart Ventricles
- Light Signal Transduction
- Myocytes, Cardiac
- Optogenetics