The transcriptome analysis of Protaetia brevitarsis Lewis larvae.

Abstract

Larvae of the pest Protaetia brevitarsis are used to treat infections in traditional Chinese medicine. However, genomic information about this non-model species is currently lacking. To better understand the fundamental biology of this non-model species, its transcriptome was obtained using next generation sequencing and then analyzed. A total of 7.62 Gb of clean reads were obtained, which were assembled into 169,087 transcripts corresponding to 142,000 annotated unigenes. These unigenes were functionally classified according to Gene Ontology (GO), euKaryotic Ortholog Groups of proteins (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. A total of 41,921 unigenes were assigned to 56 GO terms, 21,454 unigenes were divided among 26 KOG categories, and 16,368 unigenes were assigned to 32 KEGG pathways. In addition, 19,144 simple sequence repeats (SSRs) were identified. Furthermore, several kinds of natural antimicrobial peptides and proteins, 4 histones with potential antimicrobial activity, and 41 potential antimicrobial peptide sequences were identified. These data are the first reported whole transcriptome sequence of P. brevitarsis larvae, which represents a valuable genomic resource for studying this species, thus promoting the utilization of its medical potential.