Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30911168.
- Also identified by DOI 10.1038/s41586-019-1049-y and PMC identifier 6544023.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Imaging the transcriptome in situ with high accuracy has been a major challenge in single-cell biology, which is particularly hindered by the limits of optical resolution and the density of transcripts in single cells<sup>1-5</sup>. Here we demonstrate an evolution of sequential fluorescence in situ hybridization (seqFISH+). We show that seqFISH+ can image mRNAs for 10,000 genes in single cells-with high accuracy and sub-diffraction-limit resolution-in the cortex, subventricular zone and olfactory bulb of mouse brain, using a standard confocal microscope. The transcriptome-level profiling of seqFISH+ allows unbiased identification of cell classes and their spatial organization in tissues. In addition, seqFISH+ reveals subcellular mRNA localization patterns in cells and ligand-receptor pairs across neighbouring cells. This technology demonstrates the ability to generate spatial cell atlases and to perform discovery-driven studies of biological processes in situ.
Medical subject headings
- Brain
- In Situ Hybridization, Fluorescence
- RNA, Messenger
- Single-Cell Analysis
- Transcriptome