SNAC-tag for sequence-specific chemical protein cleavage.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30923372.
- Also identified by DOI 10.1038/s41592-019-0357-3 and PMC identifier 6443254.
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Abstract
Site-specific protein cleavage is essential for many protein-production protocols and typically requires proteases. We report the development of a chemical protein-cleavage method that is achieved through the use of a sequence-specific nickel-assisted cleavage (SNAC)-tag. We demonstrate that the SNAC-tag can be inserted before both water-soluble and membrane proteins to achieve fusion protein cleavage under biocompatible conditions with efficiency comparable to that of enzymes, and that the method works even when enzymatic cleavages fail.
Medical subject headings
- Enzymes
- Nickel
- Proteins