Single-cell chromatin immunocleavage sequencing (scChIC-seq) to profile histone modification.
basic_science · Level V
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- Record sourced from PubMed, PMID 30923384.
- Also identified by DOI 10.1038/s41592-019-0361-7 and PMC identifier 7187538.
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Abstract
Our method for analyzing histone modifications, scChIC-seq (single-cell chromatin immunocleavage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice by tethering to a specific antibody. Cleaved target sites are then selectively PCR amplified. We show that scChIC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells. The resulting data are used for clustering of blood cell types.
Medical subject headings
- Chromatin
- Histones
- Micrococcal Nuclease