Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30933980.
- Also identified by DOI 10.1371/journal.pone.0210374 and PMC identifier 6443234.
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Abstract
Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.
Medical subject headings
- DNA
- DNA Fragmentation
- High-Throughput Nucleotide Sequencing
- Polymerization
- Sequence Analysis, DNA