Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30936561.
- Also identified by DOI 10.1038/s41587-019-0070-x and PMC identifier 6544532.
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Abstract
Oral antibodies that interfere with gastrointestinal targets and can be manufactured at scale are needed. Here we show that a single-gene-encoded monomeric immunoglobulin A (IgA)-like antibody, composed of camelid variable single domain antibodies (VHH) fused to IgA Fc (mVHH-IgA), prevents infection by enterotoxigenic Escherichia coli (F4-ETEC) in piglets. The mVHH-IgA can be produced in soybean seeds or secreted from the yeast Pichia pastoris, freeze- or spray-dried and orally delivered within food.
Medical subject headings
- Communicable Diseases
- Gastrointestinal Diseases
- Immunoglobulin A
- Single-Domain Antibodies