The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification.
basic_science · Level V
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- Record sourced from PubMed, PMID 30951545.
- Also identified by DOI 10.1371/journal.pone.0214110 and PMC identifier 6450627.
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Abstract
Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation. Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development.
Medical subject headings
- Cell Differentiation
- Heparin-binding EGF-like Growth Factor
- Nuclear Proteins
- Placentation
- Transcription Factors