Single-cell lineage tracing by endogenous mutations enriched in transposase accessible mitochondrial DNA.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30958261.
- Also identified by DOI 10.7554/eLife.45105 and PMC identifier 6469926.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Simultaneous measurement of cell lineage and cell fates is a longstanding goal in biomedicine. Here we describe EMBLEM, a strategy to track cell lineage using endogenous mitochondrial DNA variants in ATAC-seq data. We show that somatic mutations in mitochondrial DNA can reconstruct cell lineage relationships at single cell resolution with high sensitivity and specificity. Using EMBLEM, we define the genetic and epigenomic clonal evolution of hematopoietic stem cells and their progenies in patients with acute myeloid leukemia. EMBLEM extends lineage tracing to any eukaryotic organism without genetic engineering.
Medical subject headings
- Cell Lineage
- Cytological Techniques
- DNA, Mitochondrial
- Hematopoietic Stem Cells
- Leukemia, Myeloid, Acute
- Mutation