In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30962622.
- Also identified by DOI 10.1038/s41592-019-0371-5 and PMC identifier 6592845.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Systematic investigation of the genetic interactions that influence metastatic potential has been challenging. Here we developed massively parallel CRISPR-Cpf1/Cas12a crRNA array profiling (MCAP), an approach for combinatorial interrogation of double knockouts in vivo. We designed an MCAP library of 11,934 arrays targeting 325 pairwise combinations of genes implicated in metastasis. By assessing the metastatic potential of the double knockouts in mice, we unveiled a quantitative landscape of genetic interactions that drive metastasis.
Medical subject headings
- Bacterial Proteins
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Endonucleases
- Gene Editing
- Gene Knockout Techniques
- Neoplasm Metastasis