High density DNA data storage library via dehydration with digital microfluidic retrieval.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 30979873.
- Also identified by DOI 10.1038/s41467-019-09517-y and PMC identifier 6461645.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
DNA promises to be a high density data storage medium, but physical storage poses a challenge. To store large amounts of data, pools must be physically isolated so they can share the same addressing scheme. We propose the storage of dehydrated DNA spots on glass as an approach for scalable DNA data storage. The dried spots can then be retrieved by a water droplet using a digital microfluidic device. Here we show that this storage schema works with varying spot organization, spotted masses of DNA, and droplet retrieval dwell times. In all cases, the majority of the DNA was retrieved and successfully sequenced. We demonstrate that the spots can be densely arranged on a microfluidic device without significant contamination of the retrieval. We also demonstrate that 1 TB of data could be stored in a single spot of DNA and successfully retrieved using this method.
Medical subject headings
- DNA
- Information Storage and Retrieval
- Lab-On-A-Chip Devices
- Microfluidics