A photocleavable surfactant for top-down proteomics.
basic_science · Level V
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- Record sourced from PubMed, PMID 30988469.
- Also identified by DOI 10.1038/s41592-019-0391-1 and PMC identifier 6532422.
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Abstract
We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-polyacrylamide gel electrophoresis.
Medical subject headings
- Azo Compounds
- Electrophoresis, Polyacrylamide Gel
- Proteomics
- Sodium Dodecyl Sulfate
- Surface-Active Agents