Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31011186.
- Also identified by DOI 10.1038/s41592-019-0392-0 and PMC identifier 6557128.
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Abstract
Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- Proteins
- Sequence Analysis, RNA
- Single-Cell Analysis
- Transcriptome