DNA-enabled rational design of fluorescence-Raman bimodal nanoprobes for cancer imaging and therapy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 31028250.
- Also identified by DOI 10.1038/s41467-019-09173-2 and PMC identifier 6486596.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Recently, surface-enhanced Raman scattering nanoprobes have shown tremendous potential in oncological imaging owing to the high sensitivity and specificity of their fingerprint-like spectra. As current Raman scanners rely on a slow, point-by-point spectrum acquisition, there is an unmet need for faster imaging to cover a clinically relevant area in real-time. Herein, we report the rational design and optimization of fluorescence-Raman bimodal nanoparticles (FRNPs) that synergistically combine the specificity of Raman spectroscopy with the versatility and speed of fluorescence imaging. DNA-enabled molecular engineering allows the rational design of FRNPs with a detection limit as low as 5 × 10<sup>-15</sup> M. FRNPs selectively accumulate in tumor tissue mouse cancer models and enable real-time fluorescence imaging for tumor detection, resection, and subsequent Raman-based verification of clean margins. Furthermore, FRNPs enable highly efficient image-guided photothermal ablation of tumors, widening the scope of the NPs into the therapeutic realm.
Medical subject headings
- Brain Neoplasms
- DNA
- Metal Nanoparticles
- Optical Imaging
- Ovarian Neoplasms
- Spectrum Analysis, Raman